In 2009, PacBio published a paper expanding the principle into a full-blown sequencing technique. Once again, each nucleotide was labeled with a different colored fluorophore detectable by the ZMW to determine which base had been incorporated. The fluorophores were attached such that they would be cleaved off during the chemical reaction incorporating the base into the growing DNA strand; they would then diffuse out of the ZMW so that the next fluorophore could be detected. Sequencing took place on a chip with many wells simultaneously — a different type of parallelization where each well detected a single DNA molecule undergoing the same basic chemical reaction.
Compounding move: Document which tasks can be done in parallel well. If you aren't careful, multiple agents can go do similar things, which can confuse them and make it hard to work. Some work is inherently serial, and knowing the difference will save you time on coordination.
Bias Term Projection，详情可参考Safew下载
; MOV DS, r32 (protected mode)
。关于这个话题，快连下载提供了深入分析
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